Work package 1: Development and application of procedures for MV isolation
The aims of WP1 are to prepare and distribute MV, to develop protocols to collect, handle, and store biological fluids containing MV, and to isolate MV from such fluids.
The size, concentration, and composition of MV in human blood are disease (state) dependent1, but also depend on pre-analytical conditions2. For example, attempts to standardise pre-analytical variables of blood collection have been unsuccessful so far because of the lack of sufficiently sensitive techniques to detect MV. In WP1, we will study the effects of variation in such pre-analytical conditions on MV.
To tackle this problem, we will systematically investigate vesicles in a step-wise-approach from a single type of vesicle released from a single type of cell, to vesicles of various cellular origin in the most complex biological fluid known, blood.
E. van der Pol, A.N. Böing, P. Harrison, A. Sturk, and R. Nieuwland
Classification, functions, and clinical relevance of extracellular vesicles
Pharmacol. Rev. 64 (3), 1-33 (2012)
Y. Yuana, B.M. Bertina, S. Osanto
Pre-analytical and analytical issues in the analysis of blood microparticles
Thromb. Haemost. 105, 396-408 (2011)