Work package 4: MV reference materials

In this WP, synthetic reference materials and biological reference materials will be developed and characterised as shown in the table below. The reference materials will be distributed among the JRP-Participants for measurements. Comparison measurements will be made to validate the reference materials and the procedures. Furthermore, selected reference materials will be distributed and measured in clinical hospital laboratories.

Currently, the use of optical equipment to detect MV in medical centers is rising. Therefore, in 2010, a collaborative workshop of the International Society on Thrombosis and Haemostasis consisting of over 60 prominent researchers in this upcoming field, proposed to use polystyrene beads to standardize measurements on MV using flow cytometry 1. Sice the refractive index of biological vesicles, which is estimated at 1.4, is significantly lower than the refractive index of polystyrene, MV scatter roughly 10-fold less light than polystyrene beads 2. As a result, data interpretation and comparison was cumbersome and the need for traceable reference standards with (optical) properties resembling MV is growing. Therefore, well-characterized reference standards are useful for calibrating instruments, examining the detection limits of instruments, monitoring the reproducibility of instruments used in this project and used by medical centers worldwide.

In this WP, synthetic reference materials and biological reference materials will be acquired, characterized, and distributed among the participants for extended measurements (WP2 and WP3). Comparison measurements will validate the developed procedures and the uncertainty estimations obtained in WP2 and WP3.

References

1.  R. Lacroix, S. Robert, P. Poncelet, R.S. Kasthuri, N.S. Key, F. Dignat-George
Standardization of platelet-derived microparticle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop
J. Thromb. Haemost. 8, 2571-4 (2010)
2.  W.L. Chandler, W. Yeung, J.F. Tait
A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer.
J. Thromb. Haemost. 9, 1216-24 (2011)